Periostin antibody detects the human periostin protein. This polyclonal antibody is available unconjugated or conjugated with horseradish peroxidase, phycoerythrin, FITC, and Alexa Fluor dyes for western blotting (WB), immunoprecipitation (IP), immunofluorescence, and ELISA assays.

periostin antibody , also known as osteoblast-specific factor 2 or OSF-2, is a disulfide-linked secretory protein involved in cell-cell adhesion and bone and tissue remodeling. It contains an N-terminal EMI domain rich in cysteine residues, four tandem FAS1 domains that are involved in protein-protein interactions, and a carboxyl end binding to heparin and other ECM proteins. Periostin is alternatively spliced to produce several isoforms, which vary in their biologic activity.

Validated Antibodies for Western Blot, IHC, and ELISA

Plasma periostin levels were elevated in rapid progressors versus nonprogressors of IPF and localized to areas of active fibrosis in the lungs. In addition, periostin mRNA was enriched in the lungs of IPF patients and increased in bleomycin-treated mice. Fibroblasts from IPF lungs made 3.5 times as much periostin mRNA as fibroblasts from normal lung tissue.

To test whether periostin has a role in the formation of ECM fibrils, we used a kinetic imaging program to monitor scratch wound closure in confluent monolayers of wild-type murine mesenchymal cells treated with or without varying concentrations of periostin in serum-free medium. Periostin stimulated the speed and density of wound closure in a dose-dependent manner. We further investigated periostin’s effect on PAI-1 transcription in epithelial cells by treating PAIL cells, which are mink lung epithelial cells stably transfected with a PAI-1 promoter-luciferase reporter gene. Treatment of the cells with periostin significantly increased PAI-1 transcription in a luciferase assay in a dose-dependent fashion.